Segmentation of Hoechst stained Hela-Kyoto nuclei in 2D using segmct from Example 10. A) Input image showing stained nuclei, B) after edge enhancing diffusion, C) segmentation by adaptive thresholding (prm.method = ’adth’) without splitting of cells, and D) with splitting of cells. Note that the connected nuclei are now disconnected. E) Segmentation by iterative thresholding (prm.method = ’thrs’) without splitting, and F) after splitting. Both methods are successfull.