Simple tools for assembling and searching high-density picolitre pyrophosphate sequence data

Table 2 Bases inserted due to incomplete extension.

	Forward	Reverse
TTAAAATAAAAAAAAAGA CGCAT	17	0
TTAAAATAAAAAAAAAGCGCAT	8	14
TAAAAAAATAAAAAACTA GGTTT	18	2
TAAAAAAATAAAAAACTGGTTT	11	13
TATTGTAAATAAAAAAAAAATAA TACA	18	1
TATTGTAAATAAAAAAAAAATATACA	6	15

Paired output from count with the incorrect sequence followed by the corrected sequence. In each case, incomplete extension has resulted in the insertion of a spurious base in the following flow cycle in the forward direction, but no or very few reads in the reverse sense. When the spurious base is removed, the numbers are more nearly matched in the forward and reverse directions. In the third example, this effect caused a single A to be read as AA.

ISSN: 1751-0473