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Fig. 1 | Source Code for Biology and Medicine

Fig. 1

From: FLIM-FRET analyzer: open source software for automation of lifetime-based FRET analysis

Fig. 1

Illustration of the features of FLIM-FRET analyzer. a Multistep menu for single cell segmentation (step 1), whole image lifetime calculation (step 2), average lifetime single cell calculation (step 3), and batched multi FLIM image experiments (step 4). b Fluorescence-based (white) single cell segmentations (red). c Lifetime map. d Instrument response function (blue), Lifetime raw data (red), Lifetime fitting (green). e Average lifetime calculation at the single cell level. f Batched analysis of multiple experiments and the average single cell FLIM measurement display

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