Fig. 1

Screen-shot of the graphical outputs generated by PrimerView. a PrimerView designs primers for datasets of any size and generates a JPEG format file of the alignment for each primer illustrating its position on the target sequence. The user inputted sequence name (derived from the FASTA description line) is shown as well as the base pair positions and location of the designed primer. b A PNG file is also generated by PrimerView that maps the distribution of all designed primers onto the scaled target sequence. The target sequence is represented by a number line that equates to nucleotide position. The gene name is indicated above the number line and the name of each primer is represented by its starting position in nucleotides