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Figure 5 | Source Code for Biology and Medicine

Figure 5

From: Easyworm: an open-source software tool to determine the mechanical properties of worm-like chains

Figure 5

Graphical guide indicating which measure should be used to derive the persistence length. The crosses (synthetic chains of known persistence length) and circles (experimental polymers) correspond to data points that are given in Table 1 and Additional file1: Table S1. Light blue markers represent the samples for which the most reliable calculations of persistence length are achieved by measuring < cos θ > and/or < R2 >, whereas purple markers indicate samples for which measuring < δ2 > provides the best estimation of the persistence length. Black markers indicate samples for which all measures provided reliable results. Therefore, the light blue region indicates where measures of < cos θ > and/or < R2 > should be used to provide the most reliable value for the persistence length, whereas the pink region indicates where measure of < δ2 > should be used. Note that background coloring serves as a guide only and that the frontier between light blue and pink regions (indicated by the black arrowhead) is strongly correlated to the image size available for analysis (typically, in the orders of 1–10 μm).

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