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Table 2 Quantitative comparison of volumetric segmentation accuracy between two manual observers (O 1 ,O 2 ), CELLPROFILER and CELLSEGM

From: CellSegm - a MATLAB toolbox for high-throughput 3D cell segmentation

Comparison/Coefficient

C 1

C 2

C 3

C 1,n

C 2,n

C 3,n

O1-CellSegm

0.6772

0.7701

0.7302

0.8534

0.9187

0.7685

O2-CellSegm

0.7080

0.7690

0.7992

0.8842

0.9176

0.8375

O1-CellProfiler

0.1161

0.2961

0.1439

0.2923

0.4447

0.1822

O2-CellProfiler

0.1238

0.3060

0.1608

0.3000

0.4546

0.1991

O1-O2

0.8238

0.8514

0.9617

1.0000

1.0000

1.0000

  1. The columns are the evaluation coefficients C i , i = 1, 2, 3, as described in (1) and (2). The normalized evaluation coefficients Ci,n are also presented, arising after subtracting the inter-observer variability from C i . CELLSEGM has considerably higher success rates than CELLPROFILER.
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