Segmentation of a paraffine embedded human skin biopsy. Upper row: Light microscopical image of the whole sample, visible are the layers of the epidermis and dermis, including a part of a hair follicle. Stained are CD44 (red) and p53 (brown). Middle row: One plane of a 3D confocal fluorescence image stack of CD44 (VulcanRed; white), overlaid with the segmentation from CELLSEGM (red). For visualization purposes, the contours were dilated with a structural element of one pixel radius, and then closed with a structural element of four pixel radius. Lower row: Segmentation results using CELLSEGM (no dilation and no closing here). The segmentation is essentially confined to the cells expressing the marker at the plasma membrane to a sufficient amount.