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Table 4 SAMtools options recognised by the Bio::DB:Sam#mpileup method and the symbols used to invoke them

From: Bio-samtools: Ruby bindings for SAMtools, a library for accessing BAM files containing high-throughput sequence alignments

SAMTools option description short symbol long symbol default
r limit retrieval to a region :r :region all positions
6 assume Illumina scaled quality scores :six :illumina_quals FALSE
A count anomalous read pairs scores :A :count_anomalous FALSE
B disable BAQ computation :B :no_baq FALSE
C parameter for adjusting mapQ :C :adjust_mapq 0
d max per-BAM depth to avoid excessive memory usage :d :max_per_bam_depth 250
E extended BAQ for higher sensitivity but lower specificity :E :extended_baq FALSE
G exclude read groups listed in FILE :G :exclude_reads_file FALSE
l list of positions (chr pos) or regions (BED) :l :list_of_positions FALSE
M cap mapping quality at value :M :mapping_quality_cap 60
R ignore RG tags :R :ignore_rg FALSE
q skip alignments with mapping quality smaller than value :q :min_mapping_quality 0
Q skip bases with base quality smaller than value :Q :imin_base_quality 13