Figure 2
From: DOG-SPOT database for comprehensive management of dog genetic research data
Screen shot of the UC Santa Cruz genome browser showing uploaded DOG-SPOT amplicon and marker data. The user runs a macro in DOG-SPOT that queries tables to generate text files of: all amplicon records (shown in gray), amplicon records for which sequence has been attempted but is poor quality or incomplete (orange), amplicon records with sufficient high quality sequence data (green) and discovered markers (purple). The user runs the make_bed.pl perl script in a folder with these four text files to generate the BED formatted file "bed.txt" that can then be uploaded to the genome browser. This visual overlay with UCSC tracks enables convenient assessment of candidate gene sequence coverage, completeness and the positioning of markers relative to genes.