Figure 6

A multi-sample Shivplot on RMA'd Data from the Pooling Experiment. To produce this figure, RMA normalization was applied to each level of replication separately, producing one data set per such level. Each of these data sets is represented by its own line on the common set of shivplot axes. The most striking feature of this illustration is the clear distinction between conditions which include some measure of biological variance (pools of one, two, or three animals) and those in which the inter-array variability is purely technical (pooling all animals, or technical replicates of a single animal).