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Table 1 An annotated example Gbrowse Moby workflow

From: Gbrowse Moby: a Web-based browser for BioMoby Services

Step

Screen

Data Type or Service (provider)

Notes

1

Initialization

Namespace: Global_keyword ID: apetala3

Select namespace and type in the ID

2

Search

GetAGILocusCodes (mips.gsf.de)

 

3

Invocation

AGI_LocusCode:At3g54340

 

4

Search

getEmblDNASequence (mips.gsf.de)

 

5

Invocation

CommentedDNASequence

type FASTA into the desired output object field and Re-initialize

6

Search

GenericSequence2FASTA (bioinfo.icapture.ubc.ca)

 

7

Invocation

FASTA

Switch the authority to antirrhinum.net to limit to the Snapdragon database. Switch semantic searching OFF to limit to services that specifically consume FASTA files.

8

Search

getDragonBlastText (antirrhinum.net)

 

9

Invocation

NCBI_Blast_Text

keep the authority as antirrhinum.net and resubmit

10

Search

parseDragonDBBlastText (antirrhinum.net)

 

11

Invocation

DragonDB_Sequence

Chose EM:AMDEFA

12

Search

getDragonSequenceLocus (antirrhinum.net)

 

13

Invocation

DragonDB_Gene

Chose DEF

14

Search

getDragonLocusAlleles (antirrhinum.net)

 

15

Invocation

DragonDB_Allele

Chose def-101

16

Search

getDragonSimpleAnnotatedImages (antirrhinum.net)

 

17

Invocation

SimpleAnnotatedJPEGImage

 
   

traverse back (with browser back button) to AGI_LocusCode:At3g54340

4a

Search

getGOTermsByAGIcode (atidb.org)

 

4b

Invocation

GO Term

 

4c

Search

getGOTermAssociations (bioinfo.icapture.ubc.ca)

 

4d

Invocation

GO Associations

 
   

traverse back (with browser back button) to AGI_LocusCode:At3g54340

4aa

Search

getNASC_codebyAGI_locus (arabidopsis.info)

 

4ab

Invocation

NASC_Code

note all of the cross-references, all clickable to reinitialize the browsing session

   

traverse back (with browser back button) to AGI_LocusCode:At3g54340

4aaa

Search

getAGRISTFFamilyNameByAGI (arabidopsis.med.ohio-state.edu)

 

4aab

Invocation

AGRIS Transcription Factor Family Name

 
  1. Steps in the browsing session are enumerated, sequentially. Numbers with suffixes (e.g. 4a) indicate an alternate path (a) starting from step 4. The column labeled Screen indicates which Gbrowse Moby screen is visible at any given step. The middle column indicates which Moby Service (for the Search screen) should be selected, or which output data-type (for the Initialization and Invocation screens) is being displayed on the screen. The Notes column provides browsing hints and/or observations that can be made at any given step.