Figure 4

A selection of available subroutines (magenta) and parameter settings (italic) in CELLSEGM related to the struct prm . In brackets is the supported data type. For example, to set the threshold th in the nucleus method of segmsurf, with thresholding method thrs, follow the stream of methods segmsurf →getminima →nucleus →segmct →thrs, and assign it by prm.segmsurf.getminima.nucleus.thrs.th in the parameter file. On the other hand, if segmct is called upon from the command prompt, the same parameter setting is defined by prm.thrs.th.